Real-time PCR probe optimization using design of experiments approach

Real-time PCR probe optimization using design of experiments approach

Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization. In this study, we present the use of statistical design of experiments (DOE) approach as a general guideline for probe optimization and more specifically focus...

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Bibliographic Details
Main Authors: S. Wadle, M. Lehnert, S. Rubenwolf, R. Zengerle, F. von Stetten
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Biomolecular Detection and Quantification
Subjects:
Design of experiments
Mediator probe PCR
Universal reporter
Real-time PCR
PCR optimization
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753515300139

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http://www.sciencedirect.com/science/article/pii/S2214753515300139

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