Development of a blocking ELISA based on a monoclonal antibody against a predominant epitope in non-structural protein 3B2 of foot-and-mouth disease virus for differentiating infected from vaccinated animals.

Development of a blocking ELISA based on a monoclonal antibody against a predominant epitope in non-structural protein 3B2 of foot-and-mouth disease virus for differentiating infected from vaccinated animals.

A monoclonal antibody (McAb) against non-structural protein (NSP) 3B of foot-mouth-disease virus (FMDV) (3B4B1) was generated and shown to recognize a conserved epitope spanning amino acids 24-32 of 3B (GPYAGPMER) by peptide screening ELISA. This epitope was further shown to be a unique and predomin...

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Bibliographic Details
Main Authors: Yuanfang Fu, Zengjun Lu, Pinghua Li, Yimei Cao, Pu Sun, Meina Tian, Na Wang, Huifang Bao, Xingwen Bai, Dong Li, Yingli Chen, Zaixin Liu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4219772?pdf=render

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http://europepmc.org/articles/PMC4219772?pdf=render

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