Standardization of a polymerase chain reaction (Semi Nested–PCR) to detect bovine herpesvirus type 1 in aborted fetus and semen from naturally infected cattle/ <br> Otimização da reação em cadeia pela polimerase (Semi Nested-PCR) para a detecção do herpesvírus bovino tipo 1 em fragmentos de órgãos fetais e em sêmen de bovinos naturalmente infectados
The glycoprotein D gene of bovine herpesvirus type 1 (BHV-1) was detected in clinical samples from naturally infected cattle by semi-nested polymerase chain reaction (SN-PCR). Different protocols were tested to increase the sensitivity and specificity of the technique. An association of DNA extracti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Londrina
2003-05-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2115 |