Gas Chromatographic Determination of Purines and Pyrimidines from DNA Using Ethyl Chloroformate as Derivatizing Reagent

Gas Chromatographic Determination of Purines and Pyrimidines from DNA Using Ethyl Chloroformate as Derivatizing Reagent

An analytical method has been proposed for the separation and determination of guanine, adenine, cytosine, thymine and uracil by gas chromatography (GC) following precolumn derivatization using ethyl chloroformate. The GC separation was achieved from HP-5 (30 m × 0.32 mm id) column with layer thickn...

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Bibliographic Details
Main Authors: Rafi O. Zaman Brohi, Muhammad Yar Khuhawar, Azizullah Channa, Abdul Jabbar Laghari, Kulsoom Abbasi
Format: Article
Language:English
Published: National Centre of Excellence in Analytical Chemsitry 2016-06-01
Series:Pakistan Journal of Analytical & Environmental Chemistry
Subjects:
Gas chromatography
Guanine
Adenine
Cytosine
Thymine
Ethyl chloroformate
Online Access:http://www.ceacsu.edu.pk/PDF%20file/Volume%2017/8.pdf

Internet

http://www.ceacsu.edu.pk/PDF%20file/Volume%2017/8.pdf

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