Zimography is an effective method for detection of matrix metalloproteinase 2 (MMP-2) activity in cultured human fibroblasts

Zimography is an effective method for detection of matrix metalloproteinase 2 (MMP-2) activity in cultured human fibroblasts

PURPOSE: To describe a method to characterize the gelatinase activity of cultured human periodontal fibroblasts stimulated with Pam3Cys and E. coli LPS, ligands of TLR2 and TLR4 respectively, and by centrifugation of the cultures, simulating an orthodontic force. METHODS: To study MMP-2 activity, pr...

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Bibliographic Details
Main Authors: Rodolfo Assis Lisboa, Marcus Vinícius Andrade, José Renan Cunha-Melo
Format: Article
Language:English
Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2013-03-01
Series:Acta Cirurgica Brasileira
Subjects:
Zymography
Matrix Metalloproteinase 2
Fibroblasts
Periodontal Ligament
Toll-Like Receptors
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300010

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300010

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