The nuclear lamina is a hub for the nuclear function of Jacob
Abstract Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold e...
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Online Access: | https://doi.org/10.1186/s13041-020-00722-1 |
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doaj-a7fdade68a7246cf928274c363d333d62021-01-17T12:25:52ZengBMCMolecular Brain1756-66062021-01-0114111210.1186/s13041-020-00722-1The nuclear lamina is a hub for the nuclear function of JacobSebastian Samer0Rajeev Raman1Gregor Laube2Michael R. Kreutz3Anna Karpova4RG Neuroplasticity, Leibniz Institute for NeurobiologyRG Neuroplasticity, Leibniz Institute for NeurobiologyRG Neuroplasticity, Leibniz Institute for NeurobiologyRG Neuroplasticity, Leibniz Institute for NeurobiologyRG Neuroplasticity, Leibniz Institute for NeurobiologyAbstract Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold electron microscopy and proximity ligation assay combined with STED imaging revealed preferential association of Jacob with the inner nuclear membrane where it directly binds to LaminB1, an intermediate filament and core component of the inner nuclear membrane (INM). The association with the INM is transient; it involves a functional nuclear export signal in Jacob and a canonical CRM1-RanGTP-dependent export mechanism that defines the residing time of the protein at the INM. Taken together, the data suggest a stepwise redistribution of Jacob within the nucleus following nuclear import and prior to nuclear export.https://doi.org/10.1186/s13041-020-00722-1Jacob/NsmfLamin B1CRM1Nuclear exportNMDARSynapse-to-nucleus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sebastian Samer Rajeev Raman Gregor Laube Michael R. Kreutz Anna Karpova |
spellingShingle |
Sebastian Samer Rajeev Raman Gregor Laube Michael R. Kreutz Anna Karpova The nuclear lamina is a hub for the nuclear function of Jacob Molecular Brain Jacob/Nsmf Lamin B1 CRM1 Nuclear export NMDAR Synapse-to-nucleus |
author_facet |
Sebastian Samer Rajeev Raman Gregor Laube Michael R. Kreutz Anna Karpova |
author_sort |
Sebastian Samer |
title |
The nuclear lamina is a hub for the nuclear function of Jacob |
title_short |
The nuclear lamina is a hub for the nuclear function of Jacob |
title_full |
The nuclear lamina is a hub for the nuclear function of Jacob |
title_fullStr |
The nuclear lamina is a hub for the nuclear function of Jacob |
title_full_unstemmed |
The nuclear lamina is a hub for the nuclear function of Jacob |
title_sort |
nuclear lamina is a hub for the nuclear function of jacob |
publisher |
BMC |
series |
Molecular Brain |
issn |
1756-6606 |
publishDate |
2021-01-01 |
description |
Abstract Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold electron microscopy and proximity ligation assay combined with STED imaging revealed preferential association of Jacob with the inner nuclear membrane where it directly binds to LaminB1, an intermediate filament and core component of the inner nuclear membrane (INM). The association with the INM is transient; it involves a functional nuclear export signal in Jacob and a canonical CRM1-RanGTP-dependent export mechanism that defines the residing time of the protein at the INM. Taken together, the data suggest a stepwise redistribution of Jacob within the nucleus following nuclear import and prior to nuclear export. |
topic |
Jacob/Nsmf Lamin B1 CRM1 Nuclear export NMDAR Synapse-to-nucleus |
url |
https://doi.org/10.1186/s13041-020-00722-1 |
work_keys_str_mv |
AT sebastiansamer thenuclearlaminaisahubforthenuclearfunctionofjacob AT rajeevraman thenuclearlaminaisahubforthenuclearfunctionofjacob AT gregorlaube thenuclearlaminaisahubforthenuclearfunctionofjacob AT michaelrkreutz thenuclearlaminaisahubforthenuclearfunctionofjacob AT annakarpova thenuclearlaminaisahubforthenuclearfunctionofjacob AT sebastiansamer nuclearlaminaisahubforthenuclearfunctionofjacob AT rajeevraman nuclearlaminaisahubforthenuclearfunctionofjacob AT gregorlaube nuclearlaminaisahubforthenuclearfunctionofjacob AT michaelrkreutz nuclearlaminaisahubforthenuclearfunctionofjacob AT annakarpova nuclearlaminaisahubforthenuclearfunctionofjacob |
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