Comparison of CRISPR and Marker-Based Methods for the Engineering of Phage T7

With the recent rise in interest in using lytic bacteriophages as therapeutic agents, there is an urgent requirement to understand their fundamental biology to enable the engineering of their genomes. Current methods of phage engineering rely on homologous recombination, followed by a system of sele...

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Bibliographic Details
Main Authors: Aurelija M. Grigonyte, Christian Harrison, Paul R. MacDonald, Ariadna Montero-Blay, Matthew Tridgett, John Duncan, Antonia P. Sagona, Chrystala Constantinidou, Alfonso Jaramillo, Andrew Millard
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Viruses
Subjects:
t7
Online Access:https://www.mdpi.com/1999-4915/12/2/193