Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury
Oligodendrocyte (OL) replacement is a promising treatment strategy for spinal cord injury (SCI). However, the poor survival of transplanted OLs or their precursors and inhibition of axonal regeneration are two major challenges with this approach. Our previous study showed that Schwann cells (SCs) pr...
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doaj-a849f2f3225a47cfbd8bed665e6172ca2021-03-22T12:44:33ZengElsevierNeurobiology of Disease1095-953X2016-10-0194196204Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injuryXiu-Mei Xie0Ling-Ling Shi1Lin Shen2Rui Wang3Qi Qi4Qi-Yi Wang5Lun-Jun Zhang6He-Zuo Lü7Jian-Guo Hu8Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaAnhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaAnhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaAnhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Corresponding author.Oligodendrocyte (OL) replacement is a promising treatment strategy for spinal cord injury (SCI). However, the poor survival of transplanted OLs or their precursors and inhibition of axonal regeneration are two major challenges with this approach. Our previous study showed that Schwann cells (SCs) promoted survival, proliferation, and migration of transplanted OL progenitor cells (OPCs) and neurological recovery. Remyelination is an important basis for functional recovery following spinal cord injury. It has been reported that myelin gene regulatory factor (MRF), a transcriptional regulator which specifically is expressed in postmitotic OLs within the CNS, is essential for OL maturation and CNS myelination. In the present study, we investigated whether co-transplantation of MRF-overexpressing OPCs (MRF-OPCs) and SCs could improve functional recovery in a rat model of contusional SCI. MRF overexpression had no effect on OPC survival or migration, but stimulated the differentiation of OPCs both in vitro and in vivo. Co-transplantation of MRF-OPCs and SCs increased myelination and tissue repair after SCI, leading to the recovery of neurological function. These results indicate that co-transplantation of MRF-OPCs and SCs may be an effective treatment strategy for SCI.http://www.sciencedirect.com/science/article/pii/S0969996116301577Myelin gene regulatory factorOL progenitor cellsSchwann cellsTransplantationSpinal cord injuryFunctional recovery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiu-Mei Xie Ling-Ling Shi Lin Shen Rui Wang Qi Qi Qi-Yi Wang Lun-Jun Zhang He-Zuo Lü Jian-Guo Hu |
spellingShingle |
Xiu-Mei Xie Ling-Ling Shi Lin Shen Rui Wang Qi Qi Qi-Yi Wang Lun-Jun Zhang He-Zuo Lü Jian-Guo Hu Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury Neurobiology of Disease Myelin gene regulatory factor OL progenitor cells Schwann cells Transplantation Spinal cord injury Functional recovery |
author_facet |
Xiu-Mei Xie Ling-Ling Shi Lin Shen Rui Wang Qi Qi Qi-Yi Wang Lun-Jun Zhang He-Zuo Lü Jian-Guo Hu |
author_sort |
Xiu-Mei Xie |
title |
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury |
title_short |
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury |
title_full |
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury |
title_fullStr |
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury |
title_full_unstemmed |
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury |
title_sort |
co-transplantation of mrf-overexpressing oligodendrocyte precursor cells and schwann cells promotes recovery in rat after spinal cord injury |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2016-10-01 |
description |
Oligodendrocyte (OL) replacement is a promising treatment strategy for spinal cord injury (SCI). However, the poor survival of transplanted OLs or their precursors and inhibition of axonal regeneration are two major challenges with this approach. Our previous study showed that Schwann cells (SCs) promoted survival, proliferation, and migration of transplanted OL progenitor cells (OPCs) and neurological recovery. Remyelination is an important basis for functional recovery following spinal cord injury. It has been reported that myelin gene regulatory factor (MRF), a transcriptional regulator which specifically is expressed in postmitotic OLs within the CNS, is essential for OL maturation and CNS myelination. In the present study, we investigated whether co-transplantation of MRF-overexpressing OPCs (MRF-OPCs) and SCs could improve functional recovery in a rat model of contusional SCI. MRF overexpression had no effect on OPC survival or migration, but stimulated the differentiation of OPCs both in vitro and in vivo. Co-transplantation of MRF-OPCs and SCs increased myelination and tissue repair after SCI, leading to the recovery of neurological function. These results indicate that co-transplantation of MRF-OPCs and SCs may be an effective treatment strategy for SCI. |
topic |
Myelin gene regulatory factor OL progenitor cells Schwann cells Transplantation Spinal cord injury Functional recovery |
url |
http://www.sciencedirect.com/science/article/pii/S0969996116301577 |
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