Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.

Bibliographic Details
Main Authors: Hiroki Sasaguri, Kenichi Nagata, Misaki Sekiguchi, Ryo Fujioka, Yukio Matsuba, Shoko Hashimoto, Kaori Sato, Deepika Kurup, Takanori Yokota, Takaomi C. Saido
Format: Article
Language:English
Published: Nature Publishing Group 2018-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-05262-w

Internet

https://doi.org/10.1038/s41467-018-05262-w

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