Screening for Oxidative Stress Elicited by Engineered Nanomaterials: Evaluation of Acellular DCFH Assay

The DCFH assay is commonly used for measuring free radicals generated by engineered nanomaterials (ENM), a well-established mechanism of ENM toxicity. Concerns exist over susceptibility of the DCFH assay to: assay conditions, adsorption of DCFH onto ENM, fluorescence quenching and light scattering....

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Bibliographic Details
Main Authors: Anoop K. Pal, Dhimiter Bello, Bridgette Budhlall, Eugene Rogers, Donald K. Milton
Format: Article
Language:English
Published: SAGE Publishing 2012-07-01
Series:Dose-Response
Online Access:https://doi.org/10.2203/dose-response.10-036.Pal