Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

Objectives: To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency. Methods: Cells were kept in culture for over 50 passages, foll...

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Bibliographic Details
Main Authors: Estela Cruvinel, Isabella Ogusuku, Rosanna Cerioni, Sirlene Rodrigues, Jéssica Gonçalves, Maria Elisa Góes, Juliana Morais Alvim, Anderson Carlos Silva, Vanesca de Souza Lino, Enrique Boccardo, Ernesto Goulart, Alexandre Pereira, Rafael Dariolli, Marcos Valadares, Diogo Biagi
Format: Article
Language:English
Published: SAGE Publishing 2020-10-01
Series:SAGE Open Medicine
Online Access:https://doi.org/10.1177/2050312120966456

Internet

https://doi.org/10.1177/2050312120966456

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