A GFP-lacZ bicistronic reporter system for promoter analysis in environmental gram-negative bacteria.
Here, we describe a bicistronic reporter system for the analysis of promoter activity in a variety of gram-negative bacteria at both the population and single-cell levels. This synthetic genetic tool utilizes an artificial operon comprising the gfp and lacZ genes that are assembled in a suicide vect...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3321037?pdf=render |