In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

Summary: The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligati...

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Bibliographic Details
Main Authors: Shensi Shen, Isabelle Girault, Hélène Malka-Mahieu, Caroline Robert, Stéphan Vagner
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003282