In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture

Summary: In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characte...

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Bibliographic Details
Main Authors: Simon E. Richardson, Roshanak Ghazanfari, Jyoti Chhetri, Tariq Enver, Charlotta Böiers
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001271