Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus
The nitric oxide synthase-like protein from Bacillus cereus (bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2010-01-01
|
Series: | Biochemistry Research International |
Online Access: | http://dx.doi.org/10.1155/2010/489892 |