Highly Sensitive Automated Method for DNA Damage Assessment: Gamma-H2AX Foci Counting and Cell Cycle Sorting

Highly Sensitive Automated Method for DNA Damage Assessment: Gamma-H2AX Foci Counting and Cell Cycle Sorting

Phosphorylation of the H2AX protein is an early step in the double strand break (DSB) repair pathway; therefore, phosphorylated histone (γH2AX) foci scoring is widely used as a measure for DSBs. Foci scoring is performed either manually or semi-automatically using hand-operated capturing and image a...

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Bibliographic Details
Main Authors: Anna Genescà, Laura Tusell, Marta Martín, Laia Hernández, Mariona Terradas
Format: Article
Language:English
Published: MDPI AG 2013-07-01
Series:International Journal of Molecular Sciences
Subjects:
gamma-H2AX
cell cycle
automated screening method
dosimetry
DNA damage
mammography
Online Access:http://www.mdpi.com/1422-0067/14/8/15810

Internet

http://www.mdpi.com/1422-0067/14/8/15810

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