The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains
Influenza vaccination is a common approach to prevent seasonal and pandemic influenza. Pre-existing antibodies against close viral strains might impair antibody formation against previously unseen strains–a process called original antigenic sin. The role of this pre-existing cellular immunity in thi...
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doaj-aa40b8fd1e7141a2895cefcebf19319e2020-11-24T20:45:03ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-04-011010.3389/fimmu.2019.00593440794The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza StrainsMikalai Nienen0Mikalai Nienen1Mikalai Nienen2Ulrik Stervbo3Felix Mölder4Sviatlana Kaliszczyk5Leon Kuchenbecker6Ludmila Gayova7Brunhilde Schweiger8Karsten Jürchott9Jochen Hecht10Jochen Hecht11Avidan U. Neumann12Sven Rahmann13Timm Westhoff14Petra Reinke15Petra Reinke16Andreas Thiel17Nina Babel18Nina Babel19Nina Babel20Institute for Medical Immunology, Charité University Medicine Berlin, Berlin, GermanyBerlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, GermanyLabor Berlin-Charité Vivantes GmbH, Berlin, GermanyCenter for Translational Medicine, Immunology and Transplantation, Marien Hospital Herne, Ruhr University Bochum, Herne, GermanyGenome Informatics, Institute of Human Genetics, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyCenter for Translational Medicine, Immunology and Transplantation, Marien Hospital Herne, Ruhr University Bochum, Herne, GermanyApplied Bioinformatics, Tübingen University, Tübingen, GermanyBogomolets National Medical University, Kyiv, UkraineRobert-Koch Institute, Berlin, GermanyBerlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, GermanyCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain0Universitat Pompeu Fabra (UPF), Barcelona, Spain1Institute of Environmental Medicine, German Research Center for Environmental Health, Helmholtz Zentrum München, Augsburg, GermanyGenome Informatics, Institute of Human Genetics, University Hospital Essen, University of Duisburg-Essen, Essen, Germany2Department of Internal Medicine, Marien Hospital Herne, Ruhr University Bochum, Herne, GermanyBerlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, Germany3Department of Nephrology and Intensive Care, Charité University Medicine Berlin, Berlin, GermanyBerlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, GermanyBerlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, GermanyCenter for Translational Medicine, Immunology and Transplantation, Marien Hospital Herne, Ruhr University Bochum, Herne, Germany3Department of Nephrology and Intensive Care, Charité University Medicine Berlin, Berlin, GermanyInfluenza vaccination is a common approach to prevent seasonal and pandemic influenza. Pre-existing antibodies against close viral strains might impair antibody formation against previously unseen strains–a process called original antigenic sin. The role of this pre-existing cellular immunity in this process is, despite some hints from animal models, not clear. Here, we analyzed cellular and humoral immunity in healthy individuals before and after vaccination with seasonal influenza vaccine. Based on influenza-specific hemagglutination inhibiting (HI) titers, vaccinees were grouped into HI-negative and -positive cohorts followed by in-depth cytometric and TCR repertoire analysis. Both serological groups revealed cross-reactive T-cell memory to the vaccine strains at baseline that gave rise to the majority of vaccine-specific T-cells post vaccination. On the contrary, very limited number of vaccine-specific T-cell clones was recruited from the naive pool. Furthermore, baseline quantity of vaccine-specific central memory helper T-cells and clonotype richness of this population directly correlated with the vaccination efficacy. Our findings suggest that the deliberate recruitment of pre-existing cross-reactive cellular memory might help to improve vaccination outcome.https://www.frontiersin.org/article/10.3389/fimmu.2019.00593/fullinfluenza vaccinationvaccination efficacypre-existing cross-reactive T-cellscentral memory T-cellclonotype diversity |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Mikalai Nienen Mikalai Nienen Mikalai Nienen Ulrik Stervbo Felix Mölder Sviatlana Kaliszczyk Leon Kuchenbecker Ludmila Gayova Brunhilde Schweiger Karsten Jürchott Jochen Hecht Jochen Hecht Avidan U. Neumann Sven Rahmann Timm Westhoff Petra Reinke Petra Reinke Andreas Thiel Nina Babel Nina Babel Nina Babel |
spellingShingle |
Mikalai Nienen Mikalai Nienen Mikalai Nienen Ulrik Stervbo Felix Mölder Sviatlana Kaliszczyk Leon Kuchenbecker Ludmila Gayova Brunhilde Schweiger Karsten Jürchott Jochen Hecht Jochen Hecht Avidan U. Neumann Sven Rahmann Timm Westhoff Petra Reinke Petra Reinke Andreas Thiel Nina Babel Nina Babel Nina Babel The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains Frontiers in Immunology influenza vaccination vaccination efficacy pre-existing cross-reactive T-cells central memory T-cell clonotype diversity |
author_facet |
Mikalai Nienen Mikalai Nienen Mikalai Nienen Ulrik Stervbo Felix Mölder Sviatlana Kaliszczyk Leon Kuchenbecker Ludmila Gayova Brunhilde Schweiger Karsten Jürchott Jochen Hecht Jochen Hecht Avidan U. Neumann Sven Rahmann Timm Westhoff Petra Reinke Petra Reinke Andreas Thiel Nina Babel Nina Babel Nina Babel |
author_sort |
Mikalai Nienen |
title |
The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains |
title_short |
The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains |
title_full |
The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains |
title_fullStr |
The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains |
title_full_unstemmed |
The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains |
title_sort |
role of pre-existing cross-reactive central memory cd4 t-cells in vaccination with previously unseen influenza strains |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-04-01 |
description |
Influenza vaccination is a common approach to prevent seasonal and pandemic influenza. Pre-existing antibodies against close viral strains might impair antibody formation against previously unseen strains–a process called original antigenic sin. The role of this pre-existing cellular immunity in this process is, despite some hints from animal models, not clear. Here, we analyzed cellular and humoral immunity in healthy individuals before and after vaccination with seasonal influenza vaccine. Based on influenza-specific hemagglutination inhibiting (HI) titers, vaccinees were grouped into HI-negative and -positive cohorts followed by in-depth cytometric and TCR repertoire analysis. Both serological groups revealed cross-reactive T-cell memory to the vaccine strains at baseline that gave rise to the majority of vaccine-specific T-cells post vaccination. On the contrary, very limited number of vaccine-specific T-cell clones was recruited from the naive pool. Furthermore, baseline quantity of vaccine-specific central memory helper T-cells and clonotype richness of this population directly correlated with the vaccination efficacy. Our findings suggest that the deliberate recruitment of pre-existing cross-reactive cellular memory might help to improve vaccination outcome. |
topic |
influenza vaccination vaccination efficacy pre-existing cross-reactive T-cells central memory T-cell clonotype diversity |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.00593/full |
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