A highly sensitive one-tube nested quantitative real-time PCR assay for specific detection of Bordetella pertussis using the LNA technique

Objectives: Bordetella pertussis is a highly contagious respiratory agent and is the causative pathogen of pertussis, which primarily affects children. Current diagnostic techniques for this pathogen have a variety of limitations including a long culture time, low bacterial load, and lack of specifi...

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Bibliographic Details
Main Authors: Rui-qing Zhang, Zheng Li, Gui-xia Li, Yan-qing Tie, Xin-na Li, Yuan Gao, Qing-xia Duan, Le Wang, Li Zhao, Guo-hao Fan, Xue-ding Bai, Rui-huan Wang, Zi-wei Chen, Jin-rong Wang, Yong Wu, Meng-chuan Zhao, Zhi-shan Feng, Ji Wang, Xue-jun Ma
Format: Article
Language:English
Published: Elsevier 2020-04-01
Series:International Journal of Infectious Diseases
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971220300564