Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching

Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching

Carbonate radicals (CO3·-) are generated by the bicarbonate-dependent peroxidase activity of cytosolic superoxide dismutase (Cu,Zn-SOD, SOD-1). The present work explored the use of bleaching of pyrogallol red (PGR) dye to quantify the rate of CO3·- formation from bovine and human SOD-1 (bSOD-1 and h...

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Bibliographic Details
Main Authors: Juan David Figueroa, Eduardo Fuentes-Lemus, Eva Dorta, Victoria Melin, Javiera Cortés-Ríos, Mario Faúndez, David Contreras, Ana Denicola, Beatriz Álvarez, Michael J. Davies, Camilo López-Alarcón
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Redox Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2213231719302666

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http://www.sciencedirect.com/science/article/pii/S2213231719302666

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