Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity

Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.

Bibliographic Details
Main Authors:	Yi Yu, Thomas C. Leete, David A. Born, Lauren Young, Luis A. Barrera, Seung-Joo Lee, Holly A. Rees, Giuseppe Ciaramella, Nicole M. Gaudelli
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-15887-5

id	doaj-ab50974242654c18950c59b5e285c447
record_format	Article
spelling	doaj-ab50974242654c18950c59b5e285c4472021-05-11T08:43:42ZengNature Publishing GroupNature Communications2041-17232020-04-0111111010.1038/s41467-020-15887-5Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activityYi Yu0Thomas C. Leete1David A. Born2Lauren Young3Luis A. Barrera4Seung-Joo Lee5Holly A. Rees6Giuseppe Ciaramella7Nicole M. Gaudelli8Beam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsBeam TherapeuticsCytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.https://doi.org/10.1038/s41467-020-15887-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli
spellingShingle	Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nature Communications
author_facet	Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli
author_sort	Yi Yu
title	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_short	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_full	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_fullStr	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_full_unstemmed	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_sort	cytosine base editors with minimized unguided dna and rna off-target events and high on-target activity
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-04-01
description	Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.
url	https://doi.org/10.1038/s41467-020-15887-5
work_keys_str_mv	AT yiyu cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT thomascleete cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT davidaborn cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT laurenyoung cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT luisabarrera cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT seungjoolee cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT hollyarees cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT giuseppeciaramella cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity AT nicolemgaudelli cytosinebaseeditorswithminimizedunguideddnaandrnaofftargeteventsandhighontargetactivity
_version_	1721450581982183424

Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity

Similar Items