The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Rare arginine codons AGA and AGG affect the heterologous expression of proteins in Eschericha coli. The tRNAs necessary for protein synthesis are scarce in E. coli strain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus −RIL. We evaluated in both bacterial strains the effect of these rare...

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Bibliographic Details
Main Authors: Beatriz Aguirre-López, Nallely Cabrera, Marietta Tuena de Gómez-Puyou, Ruy Perez-Montfort, Armando Gómez-Puyou
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Biotechnology Reports
Subjects:
Rare codons
Protein expression
Escherichia coli
Triosephosphate isomerase
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X16300832

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http://www.sciencedirect.com/science/article/pii/S2215017X16300832

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