Development and preliminary evaluation of a real-time polymerase chain reaction for the identification of Culicoides obsoletus sensu strictu, C. scoticus and C. montanus in the Obsoletus Complex in Italy
A real-time polymerase chain reaction (PCR) using a green fluorescence dye, followed by a comparison of derivative melting curves in the post-amplification phase, was developed to distinguish species of Culicoides within the Obsoletus Complex. The selected target sequence was internal transcribed sp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2010-06-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2010/46_2/215.pdf |