A Method for Extracting High-Quality RNA from Diverse Plants for Next-Generation Sequencing and Gene Expression Analyses

A Method for Extracting High-Quality RNA from Diverse Plants for Next-Generation Sequencing and Gene Expression Analyses

Premise of the study: To study gene expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNA<i>later</i>. Obta...

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Bibliographic Details
Main Authors: Roxana Yockteng, Ana M. R. Almeida, Stephen Yee, Thiago Andre, Colin Hill, Chelsea D. Specht
Format: Article
Language:English
Published: Wiley 2013-12-01
Series:Applications in Plant Sciences
Subjects:
cDNA library
gene expression
Illumina
RNA-Seq
RNA extraction
transcriptome
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1300070

Internet

http://www.bioone.org/doi/full/10.3732/apps.1300070

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