Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo
Objective: Panax notoginseng saponins (PNS), extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H. Chen), was recently discovered to have beneficial effects against neurological damage. This study investigated the effects of PNS on cerebral ischemia and eluc...
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2018-04-01
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doaj-ac0081a4937d4af29259910ab5349d5b2021-04-02T10:48:59ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482018-04-0152151160Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivoTiantian Yang0Qi Guo1Xiaowei Shi2Shuang Wu3Ya Li4Yikun Sun5Yizhou Zhao6Limin Chai7Yonghong Gao8Lixia Lou9Bin Dong10Lingqun Zhu11Key Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDepartment of Endocrinology, 456th Hospital of the People's Liberation Army, Jinan 250000, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; Corresponding author.Objective: Panax notoginseng saponins (PNS), extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H. Chen), was recently discovered to have beneficial effects against neurological damage. This study investigated the effects of PNS on cerebral ischemia and elucidated the molecular mechanisms underlying these effects. Methods: Middle cerebral artery occlusion rats were treated with PNS (3.6 mg/100 g or 7.2 mg/100 g per day) for 7 days, the gene of LINGO-1 was measured and the expression of protein synaptophysin, postsynaptic density protein 95, LINGO-1 and p-EGFR/p-PI3K/p-AKT were investigated. The weight and mNSS score of Sprague–Dawley rats in each group were recorded every day during the 7 days. Results: PNS promoted middle cerebral artery occlusion rats' weight and the recovery of neural function. PNS significantly decreased ischemia-induced LINGO-1 protein expression. PNS also elevated EGFR/PI3K/AKT phosphorylation levels. Conclusion: PNS promoted cerebral recovery from ischemic injury by accelerating synapse reconstruction and inhibiting the neuron growth negative regulatory protein LINGO-1 and activating the epidermal growth factor receptor (EGFR)/PI3K/AKT signaling pathway in vivo. Keywords: Panax notoginseng saponins, Cerebral ischemia, LINGO-1, EGFR, PI3K/AKThttp://www.sciencedirect.com/science/article/pii/S209575481730193X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiantian Yang Qi Guo Xiaowei Shi Shuang Wu Ya Li Yikun Sun Yizhou Zhao Limin Chai Yonghong Gao Lixia Lou Bin Dong Lingqun Zhu |
spellingShingle |
Tiantian Yang Qi Guo Xiaowei Shi Shuang Wu Ya Li Yikun Sun Yizhou Zhao Limin Chai Yonghong Gao Lixia Lou Bin Dong Lingqun Zhu Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo Journal of Traditional Chinese Medical Sciences |
author_facet |
Tiantian Yang Qi Guo Xiaowei Shi Shuang Wu Ya Li Yikun Sun Yizhou Zhao Limin Chai Yonghong Gao Lixia Lou Bin Dong Lingqun Zhu |
author_sort |
Tiantian Yang |
title |
Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo |
title_short |
Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo |
title_full |
Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo |
title_fullStr |
Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo |
title_full_unstemmed |
Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo |
title_sort |
panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating lingo-1 and activating the egfr/pi3k/akt signaling pathways in vivo |
publisher |
Elsevier |
series |
Journal of Traditional Chinese Medical Sciences |
issn |
2095-7548 |
publishDate |
2018-04-01 |
description |
Objective: Panax notoginseng saponins (PNS), extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H. Chen), was recently discovered to have beneficial effects against neurological damage. This study investigated the effects of PNS on cerebral ischemia and elucidated the molecular mechanisms underlying these effects. Methods: Middle cerebral artery occlusion rats were treated with PNS (3.6 mg/100 g or 7.2 mg/100 g per day) for 7 days, the gene of LINGO-1 was measured and the expression of protein synaptophysin, postsynaptic density protein 95, LINGO-1 and p-EGFR/p-PI3K/p-AKT were investigated. The weight and mNSS score of Sprague–Dawley rats in each group were recorded every day during the 7 days. Results: PNS promoted middle cerebral artery occlusion rats' weight and the recovery of neural function. PNS significantly decreased ischemia-induced LINGO-1 protein expression. PNS also elevated EGFR/PI3K/AKT phosphorylation levels. Conclusion: PNS promoted cerebral recovery from ischemic injury by accelerating synapse reconstruction and inhibiting the neuron growth negative regulatory protein LINGO-1 and activating the epidermal growth factor receptor (EGFR)/PI3K/AKT signaling pathway in vivo. Keywords: Panax notoginseng saponins, Cerebral ischemia, LINGO-1, EGFR, PI3K/AKT |
url |
http://www.sciencedirect.com/science/article/pii/S209575481730193X |
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