Transcriptomic and Phenotypic Analysis of a spoIIE Mutant in Clostridium beijerinckii

Transcriptomic and Phenotypic Analysis of a spoIIE Mutant in Clostridium beijerinckii

SpoIIE is a phosphatase involved in the activation of the first sigma factor of the forespore, σF, during sporulation. A ΔspoIIE mutant of Clostridium beijerinckii NCIMB 8052, previously generated by CRISPR-Cas9, did not sporulate but still produced granulose and solvents. Microscopy analysis also s...

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Bibliographic Details
Main Authors: Mamou Diallo, Nicolas Kint, Marc Monot, Florent Collas, Isabelle Martin-Verstraete, John van der Oost, Servé W. M. Kengen, Ana M. López-Contreras
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Microbiology
Subjects:
Clostridium beijerinckii NCIMB 8052
sporulation
spoIIE
ABE production
CRISPR-Cas9
RNA seq
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.556064/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.556064/full

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