Characterization of BisI Homologs

Characterization of BisI Homologs

BisI is a sequence-specific and 5-methylcytosine (m5C)-dependent restriction endonuclease (REase), that cleaves the modified DNA sequence Gm5CNGC (G indicates that the cytosine opposite to G is modified). We expressed and purified a number of BisI homologs from sequenced bacterial genomes and used I...

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Bibliographic Details
Main Authors: Shuang-yong Xu, Elena V. Zemlyanskaya, Danila A. Gonchar, Zhiyi Sun, Peter Weigele, Alexey Fomenkov, Sergey Kh Degtyarev, Richard J. Roberts
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-07-01
Series:Frontiers in Microbiology
Subjects:
Type IV restriction system
m5C-dependent restriction endonuclease
BisI family enzymes
thermostable endonuclease
Bacillus subtilis T30 methylome
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.689929/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.689929/full

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