T7 RNA polymerase‐driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis

Summary The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Bacillus subtilis is better host for a plethora of synthetic biology and biotechnology applications. Reliable and efficient systems for the transfer of synthetic DNA between E. coli and B. subti...

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Bibliographic Details
Main Authors: Mario Juhas, James W. Ajioka
Format: Article
Language:English
Published: Wiley 2017-11-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.12843