T7 RNA polymerase‐driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis
Summary The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Bacillus subtilis is better host for a plethora of synthetic biology and biotechnology applications. Reliable and efficient systems for the transfer of synthetic DNA between E. coli and B. subti...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2017-11-01
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Series: | Microbial Biotechnology |
Online Access: | https://doi.org/10.1111/1751-7915.12843 |