Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Abstract Background Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a cell-type specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling. Limited...

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Bibliographic Details
Main Authors: Siebe Loontiens, Lisa Depestel, Suzanne Vanhauwaert, Givani Dewyn, Charlotte Gistelinck, Karen Verboom, Wouter Van Loocke, Filip Matthijssens, Andy Willaert, Jo Vandesompele, Frank Speleman, Kaat Durinck
Format: Article
Language:English
Published: BMC 2019-03-01
Series:BMC Genomics
Subjects:
RNA isolation
FACS sorting
Zebrafish
RNA sequencing
Online Access:http://link.springer.com/article/10.1186/s12864-019-5608-2

Internet

http://link.springer.com/article/10.1186/s12864-019-5608-2

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