Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples

Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples

Abstract Background Owing to the large amount of host DNA in clinical samples, generation of high-quality Plasmodium falciparum whole genome sequencing (WGS) data requires enrichment for parasite DNA. Enrichment is often achieved by leukocyte depletion of infected blood prior to storage. However, le...

Full description

Bibliographic Details
Main Authors: Zalak Shah, Matthew Adams, Kara A. Moser, Biraj Shrestha, Emily M. Stucke, Miriam K. Laufer, David Serre, Joana C. Silva, Shannon Takala-Harrison
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Malaria Journal
Subjects:
Plasmodium falciparum
Malaria
Whole genome sequencing
Selective whole genome amplification
Vacuum filtration
Online Access:http://link.springer.com/article/10.1186/s12936-020-03195-8

Internet

http://link.springer.com/article/10.1186/s12936-020-03195-8

Similar Items