Development of a Novel Biosensor-Driven Mutation and Selection System via in situ Growth of Corynebacterium crenatum for the Production of L-Arginine

Development of a Novel Biosensor-Driven Mutation and Selection System via in situ Growth of Corynebacterium crenatum for the Production of L-Arginine

The high yield mutants require a high-throughput screening method to obtain them quickly. Here, we developed an L-arginine biosensor (ARG-Select) to obtain increased L-arginine producers among a large number of mutant strains. This biosensor was constructed by ArgR protein and argC promoter, and cou...

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Bibliographic Details
Main Authors: Meijuan Xu, Pingping Liu, Jiamin Chen, Anqi Peng, Taowei Yang, Xian Zhang, Zhenghong Xu, Zhiming Rao
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
biosensor
transcription factors
high-throughput screening
L-arginine
ArgR
sacB
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00175/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.00175/full

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