Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis

Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis

Summary: The combination of population and single-cell RNA sequencing analysis using human embryonic stem cell (hESC) differentiation and developmental tissues is a powerful approach to elucidate an organ-specific cellular and molecular atlas in human embryogenesis. This protocol describes (1) cardi...

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Bibliographic Details
Main Authors: Federica Santoro, Kenneth R. Chien, Makoto Sahara
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell differentiation
Cell isolation
Flow cytometry/mass cytometry
RNA-seq
Single cell
Stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000460

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http://www.sciencedirect.com/science/article/pii/S2666166721000460

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