Mechanistic and Structural Insight to an Evolved Benzoylformate Decarboxylase with Enhanced Pyruvate Decarboxylase Activity

Mechanistic and Structural Insight to an Evolved Benzoylformate Decarboxylase with Enhanced Pyruvate Decarboxylase Activity

Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are thiamin diphosphate-dependent enzymes that share some structural and mechanistic similarities. Both enzymes catalyze the nonoxidative decarboxylation of 2-keto acids, yet differ considerably in their substrate specificity. In p...

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Bibliographic Details
Main Authors: Forest H. Andrews, Cindy Wechsler, Megan P. Rogers, Danilo Meyer, Kai Tittmann, Michael J. McLeish
Format: Article
Language:English
Published: MDPI AG 2016-11-01
Series:Catalysts
Subjects:
Thiamin diphosphate
X-ray crystallography
NMR spectroscopy
enzyme evolution
Online Access:http://www.mdpi.com/2073-4344/6/12/190

Internet

http://www.mdpi.com/2073-4344/6/12/190

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