Isolation and characterization of fetal nucleated red blood cells from maternal blood as a target for single cell sequencing‐based non‐invasive genetic testing

Isolation and characterization of fetal nucleated red blood cells from maternal blood as a target for single cell sequencing‐based non‐invasive genetic testing

Abstract Purpose Although non‐invasive prenatal testing (NIPT) based on cell‐free DNA (cfDNA) in maternal plasma has been prevailing worldwide, low levels of fetal DNA fraction may lead to false‐negative results. Since fetal cells in maternal blood provide a pure source of fetal genomic DNA, we aime...

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Bibliographic Details
Main Authors: Noriko Ito, Kazuhiro Tsukamoto, Kosuke Taniguchi, Ken Takahashi, Aikou Okamoto, Hiroaki Aoki, Yuka Otera‐Takahashi, Michihiro Kitagawa, Hiroko Ogata‐Kawata, Hideaki Morita, Kenichiro Hata, Kazuhiko Nakabayashi
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:Reproductive Medicine and Biology
Subjects:
fetal nucleated red blood cell (fNRBC)
fluorescence‐activated cell sorting (FACS)
non‐invasive prenatal testing (NIPT)
single cell
whole genome amplification (WGA)
Online Access:https://doi.org/10.1002/rmb2.12392

Internet

https://doi.org/10.1002/rmb2.12392

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