Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays
Abstract Background Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of false signals associated with background matrix interferences. However, t...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-01-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13007-020-00702-5 |