A Novel Approach to Bacterial Expression and Purification of Myristoylated Forms of Neuronal Calcium Sensor Proteins

A Novel Approach to Bacterial Expression and Purification of Myristoylated Forms of Neuronal Calcium Sensor Proteins

N-terminal myristoylation is a common co-and post-translational modification of numerous eukaryotic and viral proteins, which affects their interaction with lipids and partner proteins, thereby modulating various cellular processes. Among those are neuronal calcium sensor (NCS) proteins, mediating t...

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Bibliographic Details
Main Authors: Vasiliy I. Vladimirov, Viktoriia E. Baksheeva, Irina V. Mikhailova, Ramis G. Ismailov, Ekaterina A. Litus, Natalia K. Tikhomirova, Aliya A. Nazipova, Sergei E. Permyakov, Evgeni Yu. Zernii, Dmitry V. Zinchenko
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Biomolecules
Subjects:
recoverin
guanylate cyclase activator protein 1 (GCAP1), guanylate cyclase activator protein 2 (GCAP2), neuronal calcium sensor 1 (NCS-1), neurocalcin δ (NCALD), myristoylation
N-myristoyl transferase
Online Access:https://www.mdpi.com/2218-273X/10/7/1025

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https://www.mdpi.com/2218-273X/10/7/1025

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