High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser

Abstract Here we demonstrate high-pulse-energy multiphoton microscopy (MPM) for intravital imaging of neurons and oligodendrocytes in the murine brain. Pulses with an order of magnitude higher energy (~ 10 nJ) were employed from a ytterbium doped fiber laser source at a 1-MHz repetition rate, as com...

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Bibliographic Details
Main Authors: Michael J. Redlich, Brad Prall, Edesly Canto-Said, Yevgeniy Busarov, Lilit Shirinyan-Tuka, Arafat Meah, Hyungsik Lim
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-86924-6