Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene <em>Bcl-<span style="font-variant: small-caps;">x</span><sub>L</sub></em> Combined with Rapamycin

Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene <em>Bcl-<span style="font-variant: small-caps;">x</span><sub>L</sub></em> Combined with Rapamycin

CHO cells are often used to produce monoclonal antibodies in mammalian cell expression systems. In the process of large-scale cell culture, apoptosis is related to cell survival and product quality. Over-expressing an anti-apoptotic gene to delay apoptosis and improve cell growth is one of the strat...

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Bibliographic Details
Main Authors: Yunxia Li, Xinyu Zhang, Lei Wang, Huifang Zong, Yuan Yuan, Lei Han, Xi Li, Chenxiao Xu, Jingyi Zhang, Jianwei Zhu, Baohong Zhang
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Processes
Subjects:
anti-apoptotic gene
<i>Bcl-x<sub>L</sub></i>
rapamycin
Chinese Hamster Ovary
transient gene expression technology
Online Access:https://www.mdpi.com/2227-9717/7/6/329

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https://www.mdpi.com/2227-9717/7/6/329

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