Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries

Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries

Abstract Background Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The assays are based on construction of highly diverse plasmid libraries containing two variable fragments, a region of interest (...

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Bibliographic Details
Main Authors: Evgeniya S. Omelina, Anton V. Ivankin, Anna E. Letiagina, Alexey V. Pindyurin
Format: Article
Language:English
Published: BMC 2019-07-01
Series:BMC Genomics
Subjects:
Massively parallel reporter assay (MPRA)
Emulsion PCR (ePCR)
Conventional PCR
Chimeric DNA molecules
Next-generation sequencing
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Online Access:http://link.springer.com/article/10.1186/s12864-019-5847-2

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http://link.springer.com/article/10.1186/s12864-019-5847-2

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