Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart
Dopa decarboxylase (DDC) synthesizes serotonin in the developing mouse heart where it is encoded by Ddc_exon1a, a tissue-specific paternally expressed imprinted gene. Ddc_exon1a shares an imprinting control region (ICR) with the imprinted, maternally expressed (outside of the central nervous system)...
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2021-06-01
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doaj-ae3e5048d96b4447a5c8d0554890d0bb2021-06-22T06:32:59ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-06-01910.3389/fcell.2021.676543676543Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing HeartAdam R. Prickett0Bertille Montibus1Nikolaos Barkas2Samuele M. Amante3Maurício M. Franco4Michael Cowley5William Puszyk6Matthew F. Shannon7Melita D. Irving8Melita D. Irving9Marta Madon-Simon10Andrew Ward11Reiner Schulz12H. Scott Baldwin13Rebecca J. Oakey14Department of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Clinical Genetics, Guy’s and St Thomas’ NHS Foundation Trust, London, United KingdomDepartment of Biology and Biochemistry and Centre for Regenerative Medicine, University of Bath, Bath, United KingdomDepartment of Biology and Biochemistry and Centre for Regenerative Medicine, University of Bath, Bath, United KingdomDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDepartment of Pediatrics (Cardiology), Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Medical and Molecular Genetics, King’s College London, London, United KingdomDopa decarboxylase (DDC) synthesizes serotonin in the developing mouse heart where it is encoded by Ddc_exon1a, a tissue-specific paternally expressed imprinted gene. Ddc_exon1a shares an imprinting control region (ICR) with the imprinted, maternally expressed (outside of the central nervous system) Grb10 gene on mouse chromosome 11, but little else is known about the tissue-specific imprinted expression of Ddc_exon1a. Fluorescent immunostaining localizes DDC to the developing myocardium in the pre-natal mouse heart, in a region susceptible to abnormal development and implicated in congenital heart defects in human. Ddc_exon1a and Grb10 are not co-expressed in heart nor in brain where Grb10 is also paternally expressed, despite sharing an ICR, indicating they are mechanistically linked by their shared ICR but not by Grb10 gene expression. Evidence from a Ddc_exon1a gene knockout mouse model suggests that it mediates the growth of the developing myocardium and a thinning of the myocardium is observed in a small number of mutant mice examined, with changes in gene expression detected by microarray analysis. Comparative studies in the human developing heart reveal a paternal expression bias with polymorphic imprinting patterns between individual human hearts at DDC_EXON1a, a finding consistent with other imprinted genes in human.https://www.frontiersin.org/articles/10.3389/fcell.2021.676543/fulldopa decarboxylaseknock-outimprintingheartmousehuman |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adam R. Prickett Bertille Montibus Nikolaos Barkas Samuele M. Amante Maurício M. Franco Michael Cowley William Puszyk Matthew F. Shannon Melita D. Irving Melita D. Irving Marta Madon-Simon Andrew Ward Reiner Schulz H. Scott Baldwin Rebecca J. Oakey |
spellingShingle |
Adam R. Prickett Bertille Montibus Nikolaos Barkas Samuele M. Amante Maurício M. Franco Michael Cowley William Puszyk Matthew F. Shannon Melita D. Irving Melita D. Irving Marta Madon-Simon Andrew Ward Reiner Schulz H. Scott Baldwin Rebecca J. Oakey Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart Frontiers in Cell and Developmental Biology dopa decarboxylase knock-out imprinting heart mouse human |
author_facet |
Adam R. Prickett Bertille Montibus Nikolaos Barkas Samuele M. Amante Maurício M. Franco Michael Cowley William Puszyk Matthew F. Shannon Melita D. Irving Melita D. Irving Marta Madon-Simon Andrew Ward Reiner Schulz H. Scott Baldwin Rebecca J. Oakey |
author_sort |
Adam R. Prickett |
title |
Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart |
title_short |
Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart |
title_full |
Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart |
title_fullStr |
Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart |
title_full_unstemmed |
Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart |
title_sort |
imprinted gene expression and function of the dopa decarboxylase gene in the developing heart |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-06-01 |
description |
Dopa decarboxylase (DDC) synthesizes serotonin in the developing mouse heart where it is encoded by Ddc_exon1a, a tissue-specific paternally expressed imprinted gene. Ddc_exon1a shares an imprinting control region (ICR) with the imprinted, maternally expressed (outside of the central nervous system) Grb10 gene on mouse chromosome 11, but little else is known about the tissue-specific imprinted expression of Ddc_exon1a. Fluorescent immunostaining localizes DDC to the developing myocardium in the pre-natal mouse heart, in a region susceptible to abnormal development and implicated in congenital heart defects in human. Ddc_exon1a and Grb10 are not co-expressed in heart nor in brain where Grb10 is also paternally expressed, despite sharing an ICR, indicating they are mechanistically linked by their shared ICR but not by Grb10 gene expression. Evidence from a Ddc_exon1a gene knockout mouse model suggests that it mediates the growth of the developing myocardium and a thinning of the myocardium is observed in a small number of mutant mice examined, with changes in gene expression detected by microarray analysis. Comparative studies in the human developing heart reveal a paternal expression bias with polymorphic imprinting patterns between individual human hearts at DDC_EXON1a, a finding consistent with other imprinted genes in human. |
topic |
dopa decarboxylase knock-out imprinting heart mouse human |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.676543/full |
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