Over-expression of Mycobacterium neoaurum 3-ketosteroid-Δ1-dehydrogenase in Corynebacterium crenatum for efficient bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione

Over-expression of Mycobacterium neoaurum 3-ketosteroid-Δ1-dehydrogenase in Corynebacterium crenatum for efficient bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione

Background: 3-Ketosteroid-Δ1-dehydrogenase (KSDD), a flavoprotein enzyme, catalyzes the bioconversion of 4-androstene-3,17-dione (AD) to androst-1,4-diene-3,17-dione (ADD). To date, there has been no report about characterization of KSDD from Mycobacterium neoaurum strains, which were usually employ...

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Bibliographic Details
Main Authors: Xian Zhang, Dan Wu, Taowei Yang, Meijuan Xu, Zhiming Rao
Format: Article
Language:English
Published: Elsevier 2016-11-01
Series:Electronic Journal of Biotechnology
Subjects:
Androst-1,4-diene-3,17-dione
Bioconversion
Codon optimization
Flavoprotein enzyme
Heterologous expression
Mycobacterium neoaurum
Overexpression
Recombinant Corynebacterium
Sterol catabolism
Whole-cell catalyst
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345816300999

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http://www.sciencedirect.com/science/article/pii/S0717345816300999

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