Protocol for analyzing the biosynthesis and degradation of N-glycan precursors in mammalian cells

Protocol for analyzing the biosynthesis and degradation of N-glycan precursors in mammalian cells

Summary: N-glycosylation is a fundamental post-translational protein modification in the endoplasmic reticulum of eukaryotic cells. The biosynthetic and catabolic flux of N-glycans in eukaryotic cells has long been analyzed by metabolic labeling using radiolabeled sugars. Here, we introduce a non-ra...

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Bibliographic Details
Main Authors: Yoichiro Harada, Kazuki Nakajima, Shengtao Li, Tadashi Suzuki, Naoyuki Taniguchi
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell biology
Metabolism
Protein biochemistry
Protein expression and purification
Mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100023X

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http://www.sciencedirect.com/science/article/pii/S266616672100023X

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