Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions

Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions

Evaluating the biophysical and functional nature of IgG is key to defining correlates of protection in infectious disease, and autoimmunity research cohorts, as well as vaccine efficacy trials. These studies often require small quantities of IgG to be purified from plasma for downstream analysis wit...

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Bibliographic Details
Main Authors: Ester Lopez, Nichollas E. Scott, Bruce D. Wines, P. Mark Hogarth, Adam K. Wheatley, Stephen J. Kent, Amy W. Chung
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Immunology
Subjects:
Fcγ receptors
IgG purification
protein G
melon gel
antibody
antibody dependent cellular phagocytosis (ADCP)
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.02415/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2019.02415/full

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