Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Abstract Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. Due to technic...

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Bibliographic Details
Main Authors: Yang Zhang, Chunyang Dai, Huiyan Wang, Yong Gao, Tuantuan Li, Yan Fang, Zuojun Shen, Lichang Chen, Zhaowu Chen, Xuejun Ma, Ming Li
Format: Article
Language:English
Published: BMC 2020-12-01
Series:Virology Journal
Subjects:
COVID-19
SARS-CoV-2
qRT-PCR
OSN-qRT-PCR
ddPCR
Highly sensitive
Online Access:https://doi.org/10.1186/s12985-020-01467-y

Internet

https://doi.org/10.1186/s12985-020-01467-y

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