Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies

Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies

We present solid-state NMR sample preparation and first 2D spectra of the Bacillus subtilis ATP-binding cassette transporter BmrA, a membrane protein involved in multidrug resistance. The homodimeric 130-kDa protein is a challenge for structural characterization due to its membrane-bound nature, siz...

Full description

Bibliographic Details
Main Authors: Britta eKunert, Carole eGardiennet, Denis eLacabanne, Daniel eCalles-Garcia, Pierre eFalson, Jean-Michel eJault, Beat H Meier, Penin eFrancois, Anja eBöckmann
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-06-01
Series:Frontiers in Molecular Biosciences
Subjects:
Isotope Labeling
solid-state NMR
ABC-transporter
BmrA
B. subtilis lipids
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmolb.2014.00005/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmolb.2014.00005/full

Similar Items