Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies
We present solid-state NMR sample preparation and first 2D spectra of the Bacillus subtilis ATP-binding cassette transporter BmrA, a membrane protein involved in multidrug resistance. The homodimeric 130-kDa protein is a challenge for structural characterization due to its membrane-bound nature, siz...
Main Authors: | Britta eKunert, Carole eGardiennet, Denis eLacabanne, Daniel eCalles-Garcia, Pierre eFalson, Jean-Michel eJault, Beat H Meier, Penin eFrancois, Anja eBöckmann |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2014-06-01
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Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmolb.2014.00005/full |
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