Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator

Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood. The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis)...

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Bibliographic Details
Main Authors: Esin Akgul Kalkan, Mehtap Sahiner, Dilek Ulker Cakir, Duygu Alpaslan, Selehattin Yilmaz
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2016/5176320