An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences.

BACKGROUND: cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge. Typically, quantitative expression data and sequence information are obtained for a large number of differentially expr...

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Bibliographic Details
Main Authors: Jacob Pollier, Miguel González-Guzmán, Wilson Ardiles-Diaz, Danny Geelen, Alain Goossens
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3174253?pdf=render