Protocol: Streamline cloning of genes into binary vectors in <it>Agrobacterium </it>via the Gateway<sup>® </sup>TOPO vector system
<p>Abstract</p> <p>Background</p> <p>In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-01-01
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| Series: | Plant Methods |
| Online Access: | http://www.plantmethods.com/content/4/1/4 |