Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derive...

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Bibliographic Details
Main Authors: Emmanuel Bréard, Estelle Lara, Loïc Comtet, Cyril Viarouge, Virginie Doceul, Alexandra Desprat, Damien Vitour, Nathalie Pozzi, Ann Brigitte Cay, Nick De Regge, Philippe Pourquier, Horst Schirrmeier, Bernd Hoffmann, Martin Beer, Corinne Sailleau, Stéphan Zientara
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23335964/pdf/?tool=EBI