Highly parallel genome-wide expression analysis of single mammalian cells.

Highly parallel genome-wide expression analysis of single mammalian cells.

We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs.The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for gl...

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Bibliographic Details
Main Authors: Jian-Bing Fan, Jing Chen, Craig S April, Jeffrey S Fisher, Brandy Klotzle, Marina Bibikova, Fiona Kaper, Mostafa Ronaghi, Sten Linnarsson, Takayo Ota, Jeremy Chien, Louise C Laurent, Jeanne F Loring, Sean V Nisperos, Gina Y Chen, Jiang F Zhong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3275609?pdf=render

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http://europepmc.org/articles/PMC3275609?pdf=render

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